韦柳静
发布时间:2022-02-14   访问次数:8829


韦柳静


生物工程专业:硕士生导师

生物化工专业:硕士生导师

生物与医药专业(生物工程领域):硕士生导师



韦柳静,女,博士,williamhill威廉希尔官网副研究员。2009年获得williamhill威廉希尔官网生物化工专业博士学位,并于同年加入生物反应器工程国家重点实验室系统生物技术研究组至今。2014-2015年在美国伊利诺伊大学香槟分校从事访问学者研究工作。长期致力于合成生物学、代谢工程、生物化工等领域相关研究工作。主持并承担国家重点研发计划、国家自然科学基金、上海市自然科学基金,企业合作等多个科研项目,在国内外主流期刊J Agr Food Chem, ACS Synth BiolBiotechnol BioengAppl Environ Microb, Bioresource TechnolBiotechnol Biofuels发表论文30余篇,申请发明专利十余项(授权2项)。

 

研究方向:

致力于微生物天然产物合成,发展和运用合成生物学,系统生物学和代谢工程的新方法,在模式工业微生物中进行改造,重构与调试;开发和优化生物燃料,大宗发酵产品和医药中间体的合成途径;通过生物过程放大优化,提高目标产物产量并满足市场的开发需求。

 

主持或参加的科研项目或基金:

12020-2024 国家重点研发计划新型工业微生物全基因组代谢网络模型的优化设计和构建研究2019YFA0904302)项目负责人;

22018 -2021 上海市自然科学基金基于合成生物新技术创建高效生产高能燃料的细胞工厂(18ZR1410000项目负责人;

32013- 2015国家自然科学基金青年科学基金氧化葡萄糖酸杆菌代谢机理研究及应用(31200025项目负责人;

42011 -2014上海市自然科学基金氧化葡萄糖酸杆菌对葡萄糖利用的代谢工程分析和改造(11ZR1408100)项目负责人;

52012 -2014williamhill威廉希尔官网基本科研业务费探索研究基金氧化葡萄糖酸杆菌对木糖代谢的研究项目负责人;

62011 -2012williamhill威廉希尔官网优秀青年教师科研专项基金基于13C标记的氧化葡萄糖酸杆菌代谢工程研究项目负责人;

 

教学:

基础生物学

食品微生物学实验

生命科学概论

微生物综合实验

生物工程专业英语(工程硕士)

 

代表性论文(#第一作者、*通讯作者):  

1. Liu-Jing Wei#*, Xuan Cao#, Jing-Jing Liu, Suryang Kwak, Yong-Su Jin, Wei Wang, and Qiang Hua*. Increased accumulation of squalene in engineered Yarrowia lipolytica through deletion of PEX10 and URE2. Appl Environ Microb, 2021,87(17): e00481-21. (IF: 4.792)

2. Liu-Jing Wei#*, Yu-Yue Ma#, Bo-Qian Cheng, Qi Gao, Qiang Hua*. Metabolic engineering Yarrowia lipolytica for a dual biocatalytic system to produce fatty acid ethyl esters from renewable feedstock in situ and in one-pot. Appl Microbiol Biot, 2021, 105(21-22): 8561-8573. (IF: 3.69)

3.  Liu-Jing Wei#*, Yu-Tao Zhong#, Ming-Yue Nie, Shun-Cheng Liu, Qiang Hua*.Biosynthesis of α-pinene by genetically engineered Yarrowia lipolytica from low-cost renewable feedstocks. J Agr Food Chem, 2021, 69, 275-285 (IF: 4.192, 1Top).

4.  Ling Zhang, Ming-Yue Nie, Feng Liu, Jun Chen, Liu-Jing Wei*, Qiang Hua*. Multiple gene integration to promote erythritol production on glycerol in Yarrowia lipolytica.Biotechnol Lett, 2021, 43(7), 1277-1287 (IF: 2.129).

5.  Gao Qi, Jing-Lin Yang, Xin-Ru Zhao, Shun-Cheng Liu, Zhi-Jie Liu, Liu-Jing Wei*, Qing Hua*. Yarrowia lipolytica as a metabolic engineering platform for the production of very-long-chain wax esters. J Agr Food Chem, 2020, 68:10730-10740 (IF: 4.192, 1Top)

6.  Feng Yao, Shun-Cheng Liu, Dan-Ni Wang, Zhi-Jie Liu, Qiang Hua, Liu-Jing Wei*. Engineering oleaginous yeast Yarrowia lipolytica for enhanced limonene production from xylose and lignocellulosic hydrolysate. FEMS Yeast Res, 2020, 20(6):1-9 (IF: 3.193)

7. Shun-Cheng Liu, Zhi-Jie Liu, Liu-Jing Wei*, Qiang Hua*. Pathway engineering and medium optimization for alpha-farnesene biosynthesis in oleaginous yeast Yarrowia lipolytica. J Biotechnol, 2020, 319:74-81 (IF: 3.503)

8.  Bo-Qian Cheng#, Liu-Jing Wei#*, Yu-Bei Lv, Jun Chen, Qiang Hua. Elevating limonene production in oleaginous yeast Yarrowia lipolytica via genetic engineering of limonene biosynthesis pathway and optimization of medium composition. Biotechnol Bioproc E, 2019, 24:500-506 (IF: 2.231)

9.   Yu-Ying Huang, Xing-Xing Jian, Yu-Bei Lv, Ke-Qing Nian, Qi Gao, Jun Chen, Liu-Jing Wei*, Qiang Hua. Enhanced squalene biosynthesis in Yarrowia lipolytica based on metabolically engineered acetyl-CoA metabolism. J Biotechnol,2018, 218:106-114 (IF: 3.163)

10.  Qi Gao, Xuan Cao, Yu-Ying Huang, Jun Chen, Liu-Jing Wei*, Qiang Hua*. Overproduction of fatty acid ethyl esters by the oleaginous yeast Yarrowia lipolytica through metabolic engineering and process optimization. ACS Synth Biol, 2018, 7(5): 1371-1380 (IF: 5.571)

11.  Liu-Jing Wei, Suryang Kwak, Jing-Jing Liu, Stephan Lane, Qiang Hua, Dae-Hyuk Kweon, Yong-Su Jin*. Improved squalene production through increasing lipid contents in Saccharomyces cerevisiae. Biotechnol Bioeng, 2018, 115: 1793-1800 (IF: 4.26)

12. Xuan Cao#, Liu-Jing Wei #, Jin-Yu Lin, Qiang Hua*. Enhancing linalool production by engineering oleaginous yeast Yarrowia lipolytica. Bioresource Technol. 2017, 245: 1641-1644. (IF: 5.8071Top)

13.  Xuan Cao, Yu-Bei Lv, Jun Chen, Tadayuki Imanaka, Liu-Jing Wei* and Qiang Hua*. Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction. Biotechnol Biofuels. 2016, 9:214. (IF: 5.2031Top)

14.  Xia Yang, Komi Nambou, Liujing Wei*, Qiang Hua. Heterologous production of a-farnesene in metabolically engineered strains of Yarrowia lipolytica. Bioresource Technol. 2016, 216: 1040–1048. (IF: 5.6511Top)

15.  Tinglan Li, Yuxiang Fan, Komi Nambou, Fengxian Hu, Tadayuki Imanaka, Liujing Wei*, Qiang Hua. Improvement of Ansamitocin P-3 Production by Actinosynnema mirum with Fructose as the Sole Carbon Source. Appl Biochem Biotechnol. 2015, 175:2845–2856.

16.  Liu-Jing Wei, Zhu Danni, Zhou Jilai, Zhang Jiajing, Zhu Kun, Du Liqin, Hua Q. Revealing in vivo glucose utilization of Gluconobacter oxydans 621H Δmgdh strain by mutagenesis. Microbiol Res. 2014, 169 (5-6): 469-475.

17.  Min-Hua Zhang#, Liu-Jing Wei#, Zhou Y, Du LQ, Imanaka T, Hua Q. Genetic analysis of D-xylose metabolism pathways in Gluconobacter oxydans 621H. J Ind Microbiol Biotechnol. 201340 (3-4): 379-388.

18. Liu-Jing Wei, Zhou JL, Zhu DN, Cai BY, Lin JP, Wei DZ, Hua Q. Functions of membrane-bound alcohol dehydrogenase and aldehyde dehydrogenase inthe biooxidation of alcohols in Gluconobacter oxydans DSM 2003. Biotechnol Bioproc E.2012, 17:1156-1164.

19. Lei-Fang Lu#, Liu-Jing Wei#, Zhu K, Wei DZ, Hua Q. Combining metabolic engineering and adaptive evolution to enhance the production of dihydroxyacetone from glycerol by Gluconobacter oxydans in a low-cost way. Bioresource Technol. 2012, (117) 317–324.

20. Liu-Jing Wei, YangXP, Gao KL,Lin JP,Yang SL,Hua Q, Wei DZ. Characterization of enzymes in the oxidation of 1,2-propanediol to D-(–)-lactic acid by Gluconobacter oxydans DSM 2003. Mol Biotechnology. 2010, 46:26–33.

21. Xue-Peng Yang#, Liu-Jing Wei#, Lin JP, Yin B, Wei DZ. Membrane-bound pyrroloquinoline quinone-dependent Dehydrogenase in Gluconobacter oxydans M5, responsible for product of 6-(2-hydroxyethyl) amino-6-deoxy-L-Sorbose. Appl Environ Microbiol. 200874: 5250-5253.

22.  韦柳静, 林金萍, 杨雪鹏, 魏东芝。氧化葡萄糖酸杆菌DSM 2003膜结合乙醇脱氢酶的纯化鉴定和性质研究。《食品科学》2010, 31: 164-168.

 

发明专利:

1、发明名称:一种应用静息细胞制备光学纯D-型β-羟基酸的方法,发明人:魏东芝、林金萍、魏国栋、杨雪鹏、韦柳静、周英、殷波,申请号:200810037960.X,申请日:2008523

2、发明名称:氧化葡萄糖酸杆菌基因敲除菌及其制备方法。发明人:花强,韦柳静,祝坤,卢磊芳,张敏华,柳志杰,申请号:201010228337.X,申请日:2010716

3、发明名称:一种基因工程菌株及利用该菌株生产二羟基丙酮的方法。发明人:花强,韦柳静,卢磊芳,张敏华,蒿珍珍,张加奇,马娜,章辉,潘鹏程。申请号:20111049078.5,申请日:20111209

4、发明名称:一种DAHP合酶的异源可溶性表达方法及其重组载体和基因工程菌。发明人:花强,韦柳静,马娜,范宇翔,刘念念,周燕,张婕,李婷兰。申请号:201110368962.9,申请日:20111118日。

5、发明名称:一种产柠檬烯的解脂耶氏酵母基因工程菌及其应用。发明人:花强,韦柳静,曹选,吕预备,陈骏。授权号ZL201610817882.X授权公告日:20210305

6、发明名称:一种产脂肪酸乙酯的解脂耶氏酵母基因工程菌及其应用。发明人:花强,韦柳静,高琪,曹选,陈骏。授权号:ZL20161081886.7,授权公告日:20201127

7、发明名称:一种产芳樟醇的解脂耶氏酵母基因工程菌及其应用。发明人:花强,韦柳静,曹选,林嘉宇。申请号: 201710419622.1,申请日:2017606

8、发明名称:一种解脂耶氏酵母生产脂肪酸乙酯的方法及其发酵培养基。发明人:花强,程博谦,韦柳静,高琪,陈骏,刘顺成。申请号:201810509755.2,申请日:2018524

9、发明名称:一种产β-胡萝卜素的解脂耶氏酵母基因工程菌及其应用。发明人:花强,张鑫锎,韦柳静,汪丹妮,陈骏。申请号:202010107322.1,申请日:2020221

10、发明名称:一种产柠檬烯的解脂耶氏酵母基因工程菌及其应用。发明人:韦柳静,钟驭涛,花强,姚丰,吕预备。申请号:202010816695.6,申请日:2020814

11发明名称:一种解脂耶氏酵母基因工程菌及其应用。发明人:花强,韦柳静,高琪,杨敬林,赵鑫茹,陈骏。申请号:202010909142.5,申请日:202092

12、发明名称:一种产α-蒎烯的解脂耶氏酵母基因工程菌。发明人:韦柳静,钟驭涛,花强,聂明月,陈骏。申请号:202011361363.X,申请日:20201127

13、发明名称:一种产玉米黄质的解脂耶氏酵母基因工程菌及其构建方法和应用。发明人:花强,韦柳静,马雨悦,陈骏。申请号:202110534354.4,申请日:2021922

 

联系方式:

电话021-6425214713916873663

邮箱weiliujing@ecust.edu.cn

通讯地址:williamhill威廉希尔官网生物反应器国家重点实验室181205

上海市徐汇区梅陇路130williamhill威廉希尔官网

邮编:200237