刘亚京 博士

生物学专业：硕士生导师

  个人简介

  williamhill威廉希尔官网，特聘副研究员。2020年1月毕业于上海科技大学遗传学专业，获理学博士学位。2020年5月开始在中科院神经科学研究所博士后研究。曾获得上海市“超级博士后”资助。迄今以第一/共一作者身份已在Nature、Nature Biotechnology、Nature Communications、EMBO Journal、Molecular Therapy等国际权威期刊发表SCI论文9篇，已申请多项发明专利（国内专利2项，国际PCT专利1项），主持国家自然科学基金青年科学基金项目。

研究方向

基因编辑和表观遗传编辑

1. 基因编辑技术的开发与应用

2. 表观遗传编辑技术的开发与应用

3. CHO和293工程细胞的优化和改造

研究生招生

从事基因编辑工具的开发和应用；针对靶点明确的遗传疾病，尤其是无特效药的罕见病（天使综合征，亨廷顿舞蹈征等），开发具有治疗潜力的基因治疗药物。现有主要研究方向包括：1）等位基因差异化DNA甲基化编辑工具的开发；2）持久的定点基因激活系统的开发；3）底盘细胞CHO和293的优化和改造。研究领域涉及生物化学与分子生物学、细胞生物学、遗传学、合成生物学、神经生物学等。真诚欢迎对基因编辑相关生物学研究充满热情的学生加入我们的团队。

主持或参加的科研项目或基金：

(1)    国家自然科学基金委员会, 青年科学基金项目, 82101552, 定点DNA甲基化成体治疗天使综合征, 2022-01-01 至 2024-12-31, 30万元, 在研, 主持

代表性论文：  

1.  Liu, Y., Lou, S., Li, J., Liu, Y., Huang, S., Wei, Y., Liu, J., Lv, R., Tang, J., Shen, Z., Sun, Y., Huang, X., Xiong, Z., Yang, H., & Zhou, C. Epigenetic editing alleviates Angelman syndrome phenotype in mice by unsilencing paternal Ube3a. Cell Discovery2024, 10: 97. https://doi.org/10.1038/s41421-024-00727-3

2.  Liu, Y., Zhou, C., Huang, S., Dang, L., Wei, Y., He, J., Zhou, Y., Mao, S., Tao, W., Zhang, Y., Yang, H., Huang, X., & Chi, T. (2020). A Cas-embedding strategy for minimizing off-target effects of DNA base editors. Nature Communications 2020, 11, 6073. https://doi.org/10.1038/s41467-020-19690-0

3.  Liu, Y., Mao, S., Huang, S., Li, Y., Chen, Y., Di, M., Huang, X., Lv, J., Wang, X., Ge, J., Shen, S., Zhang, X., Liu, D., Huang, X., & Chi, T. REPAIR x, a specific yet highly efficient programmable A > I RNA base editor. The EMBO Journal 2020, 39: e104748. https://doi.org/10.15252/embj.2020104748

4.   Liu, Y., Li, J., Zhou, C., Meng, B., Wei, Y., Yang, G., Lu, Z., Shen, Q., Zhang, Y., Yang, H., & Qiao, Y. Allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci using Staphylococcus aureus Cas9 (SaCas9). Science Bulletin 2019, 64: 1592-1600, https://doi.org/10.1016/j.scib.2019.08.023

5.  Li, G., Liu, Y., Zeng, Y., Li, J., Wang, L., Yang, G., Chen, D., Shang, X., Chen, J., Huang, X., & Liu, J. Highly efficient and precise base editing in discarded human tripronuclear embryos. Protein and Cell 2017, 8: 776–779. https://doi.org/10.1007/s13238-017-0458-7

6.  Li, X., Wang, Y., Liu, Y., Yang, B., Wang, X., Wei, J., Lu, Z., Zhang, Y., Wu, J., Huang, X., Yang, L., & Chen, J. Base editing with a Cpf1–cytidine deaminase fusion. Nature Biotechnology 2018, 36: 324-327. https://doi.org/10.1038/nbt.4102

7. Mao, S., Liu, Y., Huang, S., Huang, X., & Chi, T. (2019). Site-directed RNA editing (SDRE): Off-target effects and their countermeasures. Journal of Genetics and Genomics 2019, 46: 531–535. https://doi.org/10.1016/j.jgg.2019.11.005

8.   Wei, Y., Zhou, Y., Liu, Y., Ying, W., Lv, R., Zhao, Q., Zhou, H., Zuo, E., Sun, Y., Yang, H., & Zhou, C. Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos. Protein and Cell 2021, 12: 741–745. https://doi.org/10.1007/s13238-021-00824-z

9.  Zhou, C., Sun, Y., Yan, R., Liu, Y., Zuo, E., Gu, C., Han, L., Wei, Y., Hu, X., Zeng, R., Li, Y., Zhou, H., Guo, F., & Yang, H. Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Nature 2019.571: 275-278. https://doi.org/10.1038/s41586-019-1314-0

10.  Li, J., Shen, Z., Liu, Y., Yan, Z., Liu, Y., Lin, X., Tang, J., Lv, R., Geng, G., Xiong, Z. Q., Zhou, C., & Yang, H. A high-fidelity RNA-targeting Cas13 restores paternal Ube3a expression and improves motor functions in Angelman syndrome mice. Molecular Therapy. Molecular Therapy 2023. 31: 2286-2295. https://doi.org/10.1016/j.ymthe.2023.02.015

联系方式：

上海市徐汇区梅陇路130号williamhill威廉希尔官网

邮编：200237

办公室：阿华楼102室

邮箱：liuyajing@ecust.edu.cn