周勉
生物学专业：博士生导师/硕士生导师
生物化工专业：博士生导师
生物工程专业：硕士生导师
生物与医药专业（生物工程领域）：博士生导师/硕士生导师
周勉，博士，副教授。2009年毕业于南京大学生命科学院，获理学学士学位。2014年于美国德克萨斯大学西南医学中心（UT Southwestern Medical Center at Dallas）获博士学位，代表性研究成果发表在Nature主刊，Molecular Microbiology，EMBO Reports, Scientific Reports等期刊上。2014年12月加入williamhill威廉希尔官网，先后入选“上海市浦江人才计划（2015）”、“上海市晨光计划（2015）”、“上海市青年科技启明星计划（2019）”等人才项目，williamhill威廉希尔官网“青年英才引进与培育计划（2015、2019）”，担任国家重点研发计划子课题负责人，获国家自然科学基金青年项目和williamhill威廉希尔官网青年教师探索研究基金资助。
研究方向：
本课题组致力于研究基因密码子偏好调控基因转录和蛋白质翻译的分子机制，以及在此理论研究基础上的底盘细胞构建和表达系统改造。具体研究方向包括：
1. 密码子偏好影响基因转录的机制研究，包括基因组表观遗传形态的改变、RNA稳定性的改变以及转录活跃性改变；
2. 密码子偏好影响蛋白质翻译的机制研究，包括对翻译速度、翻译效率及蛋白质折叠和结构的影响研究；
3. 密码子偏好分子机制研究基础上的表达系统改造，通过遗传学、分子生物学、生物信息学等方法设计重组蛋白的密码子优化策略，目的导向性构建底盘细胞，优化重组蛋白表达。
代表性SCI 论文：
1. Mian Zhou, Jinhu Guo, Joonseok Cha, Michael Chae, She Chen, Jose Barral, Matthew Sachs, Yi Liu*. Non-optimal codon usage affects expression, structure and function of clock protein FRQ. 2013 Nature, 495(7439): 111-115.
2. Joonseok Cha, Mian Zhou, Yi Liu*. CATP is a critical component of the Neurospora circadian clock by regulating the nucleosome occupancy rhythm at the frequency locus. 2013 EMBO reports, 14(10): 923-930.
3. Mian Zhou1, Tao Wang1, Jingjing Fu, Guanghua Xiao, Yi Liu*. Non-optimal codon usage influences protein structure in intrinsically disordered regions. Molecular Microbiology 2015, 97(5): 974~987.
4. Wei Shen, Ying Xue, Yiqi Liu, Chuixing Kong, Xiaolong Wang, Mengmeng Huang, Menghao Cai, Xiangshan Zhou, Yuanxing Zhang, Mian Zhou*. A novel methanol-free Pichia pastoris system for recombinant protein expression. Microbial Cell Factories 2016, 15: 178.
5. Xiaolong Wang, Qi Wang, Jinjia Wang, Peng Bai, Lei Shi, Wei Shen, Mian Zhou, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. Mit1 transcription factor mediates methanol signaling and regulates alcohol oxidase 1 promoter in Pichia pastoris. Journal of Biological Chemistry 2016, 291(12): 6245~6261.
6. Zhipeng Zhou1, Yunkun Dang1, Mian Zhou, Liande Li, Chien-Hung Yu, Jingjing Fu, She Chen, Yi Liu*. Codon usage is an important determinant of gene expression levels largely through its effects on transcription. Proceedings of the National Academy of Sciences of the United States of America 2016, 113(41):E6117-E6125.
7. Jingjing Fu, Katherine Murphy, Mian Zhou, Ying Li, Vu Lam, Christine Tabuloc, Joanna Chiu*, Yi Liu*. Codon usage affects the structure and function of the Drosophila circadian clock protein PERIOD. Genes and Development 2016, 30(15):1761-75.
8. Wei Shen, Chuixing Kong, Ying Xue, Yiqi Liu, Menghao Cai, Yuanxing Zhang, Tianyi Jiang, Xiangshan Zhou* and Mian Zhou*. Kinase screening in Pichia pastoris identified promising targets involved in cell growth and Alcohol Oxidase 1 promoter (PAOX1) regulation. PLoS One 2016, online.
9. Ning Xu, Jinxiang Zhu, Qiaoyun Zhu, Yanzi Xing, Menghao Cai, Tianyi Jiang, Mian Zhou* and Yuanxing Zhang. Identification and characterization of novel promoters for recombinant protein production in yeast Pichia pastoris. Yeast 2018, 35: 379-385.
10. Zhen Yang, Xiaohui Zhou, Yue Ma, Mian Zhou, Matthew K. Waldor, Yuanxing Zhang and Qiyao Wang*. Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus. Environmental Microbiology 2018, Epub ahead of print.
11. Zhipeng Zhou, Yunkun Dang, Mian Zhou, Haiyan Yuan and Yi Liu*. Codon usage biases co-evolve with transcription termination machinery to suppress premature cleavage and polyadenylation. Elife 2018, 7: e33569.
12. Jinxiang Zhu, Ruiqing Gong, Qiaoyun Zhu, Qiulin He, Ning Xu, Yichun Xu, Menghao Cai, Xiangshan Zhou, Yuanxing Zhang and Mian Zhou*. Genome-wide determination of gene essentiality by transposon insertion sequencing in yeast Pichia pastoris. Scientific Reports 2018, 8(1): 10223.
13. Jinxiang Zhu, Qiaoyun Zhu, Ruiqing Gong, Qin Xu, Menghao Cai, Tianyi Jiang, Xiangshan Zhou, Mian Zhou* and Yuanxing Zhang. PiggyBac transposon-mediated mutagenesis and application in yeast Komagataella phaffii. Biotechnology Letters 2018, 40(9-10): 1365-1376.
14. Xiaoyue Sun, Wei Shen, Yanyun Gao, Menghao Cai, Mian Zhou* and Yuanxing Zhang. Heterologous expression and purification of a marine alginate lyase in Escherichia coli. Protein Expression and Purification 2019, 153: 97-104.
15. Yu Han, Lifan Wei, Jingfan Xiao, Yuanxing Zhang, Qiyao Wang and Mian Zhou. Identification and study of InV as an Inverse Autotransporter family representative in Edwardsiella piscicida. Archives of Microbiology 2020, 202(5):1107-1116.
16. Yanzi Xing, Ruiqing Gong, Yichun Xu, Kunshan Liu and Mian Zhou. Codon usage bias affects α-amylase mRNA level by altering RNA stability and cytosine methylation patterns in Escherichia coli. Canadian Journal of Microbiology 2020, 66(9):521-528.
17. Yichun Xu1, Kunshan Liu1, Yu Han, Yanzi Xing, Yuanxing Zhang, Qiuying Yang, Mian Zhou. Codon usage bias regulates gene expression and protein conformation in yeast expression system P. pastoris. Microbial Cell Factories 2021, 20: 91.
18. 陈溥，陈溥，李安娜，汤岩松，龙浩雨，刘坤山，周勉，甲型流感H3抗原在毕赤酵母系统中的优化表达与纯化，激光生物学报，2021，30 (5): 422-428.
19. Kunshan Liu, Yaqi Ouyang, Ru Lin, Chenyu Ge, Mian Zhou. Strong negative correlation between codon usage bias and protein structural disorder impedes protein expression after codon optimization. Journal of Biotechnology 2022,343: 15-24.
*，通讯作者
联系方式：
地址：上海市梅陇路130号williamhill威廉希尔官网实验八楼415（办公室），406（实验室）
电话：021-64252257
邮件：mianzhou@ecust.edu.cn